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Blood Component That Turns Anthrax Bacteria Virulent Identified




Scientists have discovered the key chemical that signals Bacillus anthracis, the bacterium that causes anthrax, to become lethal. This finding opens up new avenues of exploration for the development of treatments for bacterial infections.


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Handheld nanotechnology device detects cocaine, marijuana in saliva
Royal Philips Electronics has developed a handheld device that uses nanotechnology to detect marijuana, cocaine, Speed and other drugs in saliva within 2 minutes.
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Water Transport in Resin-modified Glass-ionomer Dental Cement

Water uptake and water loss have been studied in a commercial resin-modified glass-ionomer cement, Fuji II LC, under a variety of conditions. Uptake was generally non-Fickian, but affected by temperature. At room temperature, the equilibrium water uptake values varied from 2.47 to 2.78% whereas at low temperature (12°C), it varied from 0.85 to 1.18%. Cure time affected uptake values significantly. Water uptake was much lower than in conventional glass-ionomer restorative cements exposed to water vapor. Loss of water under desiccating conditions was found to be Fickian for the first 5 h loss at both 22 and 12°C. Diffusion coefficients were between 0.45 and 0.76 x 10 -7 cm2/s, with low temperature diffusion coefficients slightly greater than those at room temperature. Plotting water loss as percentage versus s-1/2 allowed activation energies to be determined from the Arrhenius equation and these were found to be 65.6, 79.8, and 7.7 kJ/mol respectively for 30, 20, and 10 s cure times. The overall conclusion is that the main advantage of incorporating HEMA into resin-modified-glass-ionomers is to alter water loss behavior. Rate of water loss and total amount lost are both reduced. Hence, resin-modified glass-ionomers are less sensitive to water loss than conventional glass-ionomers.

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Pomology: Fruit Cultivation and Production
Provides links to websites and research articles in the applied life science pomology. The site focusses on the numerous scientific and commercial aspects of the cultivation and production of temperate, subtropical and tropical fruits.
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Effect of stand density on oak regeneration in flood plain forests in Lower Silesia, Poland

The paper describes the structure, growth and vitality of natural oak regeneration in relation to stand density. To gain a better understanding of regeneration strategies, the investigation was carried out in oak forest flood plains in Poland, which were flooded in the summer of 1997. To study the stand structure and regeneration, 108 circular plots were established in stands managed using the shelterwood system. In addition, five gaps were chosen in a stand managed using the group cutting system (Legnica forest district). The results of the study showed that oak regenerated spontaneously in all stands. The abundance and vitality of oak seedlings and saplings were very high. The number of oak seedlings and saplings significantly depended on stand density. The greatest maximum height was found in gaps (146.0 cm) and in a stand with open canopy (88.9 cm) in Wolów. In Wolów and Miekinia, the shortest oaks grew in the worst light conditions. To regenerate naturally, oak stands in preparatory cutting should be reduced in the number of trees per hectare to ~150 and decreased in basal area to ~26 m2 ha–1. The group cutting system can be used in oak stands growing on flood plain sites, but there is the danger of ground vegetation development in openings.

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Introduction to bleeding
Identifying if it is a minor or severe wound
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Development and optimization of an internally controlled dried blood spot assay for surveillance of human immunodeficiency virus type-1 drug resistance
Objectives

We present the evaluation of a methodology for the genotypic assessment of human immunodeficiency virus type-1 (HIV-1) drug resistance, optimized for use with dried blood spots (DBS).

Methods

The ability to generate HIV-1 protease (PR) and reverse transcriptase (RT) contiguous amplicons and nucleotide sequences from DBS was evaluated. Different collection matrices and extraction methodologies were compared. The relative subtype sensitivity of the amplification strategy was assessed using a comprehensive panel of plasmids representing A–H subtypes. A panel of DBS and plasma specimens was subjected to HIV genotyping. Sequences generated from each sample type were compared.

Results

Extensive replicate testing revealed most sensitivity with the use of 903 filter paper and silica/guanidine extraction, which had an estimated 95% inclusivity endpoint of 1542 proviral copies/mL, as compared with 21 573 proviral copies/mL for the FTA system. All HIV-1 group M subtypes analysed—with the exception of subtypes A2, AE, AG, F and H—had a relative sensitivity of ≤10 plasmid copies/PCR reaction. The PCR was multiplexed to include amplification of a human housekeeping gene to monitor the integrity of the human genomic DNA. Using a panel of clinical samples, we demonstrated the ability to amplify and sequence from 83% (n = 10) in the PR region and 100% (n = 12) in the RT region, of samples with detectable viral load. All specimens with an HIV-1 RNA load ≥1000 copies/mL were successfully amplified and sequenced. Twelve specimens had pol genotyping from both plasma and DBS samples. Sequence analysis and drug resistance interpretation revealed that 10 (83%) provided concordant drug resistance interpretation.

Conclusions

Our results demonstrate that the technique is appropriate for surveillance of drug resistance in untreated individuals and those with virological failure on therapy.

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